Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) yog ib qho kev tshawb pom tshiab porcine enteric pathogenic tus kabmob coronavirus uas tuaj yeem ua rau muaj dej raws plab hauv cov menyuam yug tshiab thiab ua rau muaj kev puas tsuaj loj rau kev lag luam npua.Tam sim no, tsis muaj txoj hauv kev tsim nyog los ntsuas qhov ua tau zoo ntawm SADS-CoV kab mob thiab tshuaj tiv thaiv, yog li muaj qhov xav tau ceev kom siv cov tshuaj tiv thaiv kab mob sib txuas nrog cov tshuaj tiv thaiv kab mob (ELISA) kom ua rau qhov tsis txaus no.Ntawm no, ib qho plasmid recombinant qhia SADS-CoV spike (S) protein fused nrog tib neeg IgG Fc domain tau tsim los tsim cov kab mob baculovirus recombinant thiab qhia hauv HEK 293F hlwb.S-Fc protein yog purified nrog protein G resin thiab khaws cov reactivity nrog anti-tib neeg Fc thiab anti-SADS-CoV antibodies.Tom qab ntawd S-Fc protein tau siv los tsim kev tsis ncaj ncees ELISA (S-iELISA) thiab ua kom zoo dua cov tshuaj tiv thaiv ntawm S-iELISA.Raws li qhov tshwm sim, los ntawm kev txheeb xyuas tus nqi OD450nm ntawm 40 SADS-CoV negative sera tau lees paub los ntawm immunofluorescence assay (IFA) thiab Western blotting, tus nqi txiav tawm tau txiav txim siab yog 0.3711.Tus coefficient of variation (CV) ntawm 6 SADS-CoV positive sera nyob rau hauv thiab nruab nrab ntawm kev khiav ntawm S-iELISA yog tag nrho tsawg dua 10%.Qhov kev kuaj hla-reactivity tau pom tias S-iELISA tsis muaj qhov cuam tshuam nrog lwm cov kab mob porcine sera.Tsis tas li ntawd, raws li kev tshawb pom ntawm 111 kev kuaj ntshav kuaj ntshav, tag nrho qhov sib npaug ntawm IFA thiab S-iELISA yog 97.3%.Cov kab mob neutralization kuaj nrog 7 qhov sib txawv OD450nm qhov tseem ceeb ntawm cov ntshav tau pom tias OD450nm tus nqi kuaj pom los ntawm S-iELISA tau muaj kev cuam tshuam zoo nrog kev kuaj tus kab mob neutralization.Thaum kawg, S-iELISA tau ua tiav ntawm 300 npua ua liaj ua teb cov ntshav kuaj.Cov khoom siv lag luam ntawm lwm cov kab mob porcine enteroviruses tau pom tias IgG qhov zoo ntawm SADS-CoV, TGEV, PDCoV thiab PEDV yog 81.7%, 54%, thiab 65.3%, feem., 6 % , ua.Cov txiaj ntsig tau pom tias S-iELISA yog qhov tshwj xeeb, rhiab, thiab rov tsim dua tshiab, thiab tuaj yeem siv los txheeb xyuas tus kab mob SADS-CoV hauv kev lag luam npua.Kab lus no tiv thaiv los ntawm kev cai lij choj.tag nrho cov cai.
Post lub sij hawm: Jun-22-2021